The flow through a thin sharp edged orifice plate is given by:
Flow in CFM = 13.55 x square root of pressure x diameter in inches squared.
Pressure drop across the orifice being in inches of water.
Here is a worked example of a two inch diameter orifice plate with sixteen inches of measured water pressure drop.
CFM = 13.55 x (square root of 16") x (2" squared)
CFM = 13.55 x 4 x 4
CFM = 216.8
This formula gives very good results, but it assumes that the air upstream of the orifice is completely "dead" undisturbed air.
If the upstream air is highly turbulent, it will not flow so readily through the orifice. A high velocity air stream pointed at the orifice (like with an air hose !) will not give accurate repeatable results either.
A flat, thin sharp edged orifice placed over the test hole of your flow bench will give consistent results where undisturbed room air is drawn unobstructed into the orifice. The same identical orifice fitted into the interior of the bench and used as a measurement orifice, may not perform the same, because the air entering the orifice will most likely be disturbed.
"Thin" and "sharp edged" are relative terms. A three inch hole punched into a piece of sheet metal would be considered thin and sharp edged. A drilled 1/8 inch hole through the same gauge of metal may be neither. The dimensions and hole finish are relative to material thickness. But with care, a suitably thin and sharp edged orifice is not difficult to make in any required size.
*Hint* When drilling an orifice, or boring a hole with a lathe, clamping three sheets of material tightly together, and then using just the middle sheet will usually give excellent results.